![]() ![]() Forthcoming studies are utilizing human nerve samples to verify aforementioned target genes. Novel and established genes upregulated within repair SCs are presented, yielding new potential therapeutic targets to prolong the repair SC phenotype and enhance nerve regeneration. ![]() In situ hybridization verified tissue expression of targets obtained in silico.Ĭharacterization of the murine facial nerve cellulome with scRNAseq is described for the first time. Known and novel genes expressed by repair SCs and resident cells of the peripheral nerve were identified. ![]() The Chromium X is compatible will all 10x Genomics chemistry and reagent kits allowing single-cell gene expression, immune profiling, ATAC chromatin accessibility and targeted gene expression. The peripheral nerve cellulome in healthy and injured murine facial nerve is characterized. Chromium X is the flagship instrument for all things single-cell from 10x Genomics. Tissue expression of identified gene targets was assessed with in situ hybridization. Differential gene expression analysis was utilized to identify novel transcriptional signatures. The suspension was processed through the 10X Chromium scRNAseq platform and sequencing of over 5,000 cells per condition was performed. Five days post-injury, distal nerve segments were harvested and dissociated into a single-cell suspension. Sox10-venus mice labeling Schwann cells underwent unilateral facial nerve transection. Herein, we employ single-cell RNA sequencing (scRNAseq) to characterize the peripheral nerve cellulome and delineate transcriptional states of Schwann cells transitioning from mature to repair phenotypes. However, the complex signaling that produces repair SCs requires further analysis to develop therapeutic tools to enhance regeneration through promotion and maintenance of the repair SC phenotype. How should the 10x Genomics gaskets and chips that have been used to run samples containing infectious agents be disposed We recommend discarding the used gaskets and chips according to applicable biosafety guidelines of your institution. Upregulation of transcription factor cJun is critical to the transdifferentiation of myelinating and non-myelinating Schwann cells (SCs) into repair SCs after peripheral nerve injury. of Otolaryngology, Harvard Medical School / Massachusetts Eye and Ear, Boston, MA Join us to learn how 10x Genomics Chromium Single Cell 5 Barcode Enabled Antigen Mapping (BEAM) empowers rapid discovery of antigen-specific B-cell. Eye and Ear/ Harvard Medical School, Boston, MA, (2)Boston Children's Hospital, Boston, MA, (3)Harvard Chan School of Public Health, Boston, MA, (4)Facial Nerve Center - Dept. Suresh Mohan, MD 1, Alan Tenney, PhD 2, Zhu Zhuo, PhD 3, Brandon Pratt, BS 2, Thomas Collins, BS 2, Alon Gelber, BS 2, Shannan J Ho Sui, PhD 3, Tessa A Hadlock, MD 4, Nate Jowett, MD 4 and Elizabeth C Engle, MD 2, (1)Mass. Characterizing Schwann cell transcriptional states in peripheral nerve regeneration with single-cell RNA sequencing ![]()
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